This study aims to demonstrate the inhibitory effect of local isolates of the  Bacillus against  F. oxysporum  wilt of tomatoes in vitro, and in experimental field conditions in Morogoro region. Fusarium wilt is considered one of the most important disease of crops and especially vegetables in fields and greenhouses. The disease is characterized by wilting of plants, yellowing of leaves and reduced to even total loss of crop yield. In Tanzania, Fusarium wilt has been reported in many crops, however, Foxysporum f.sp lycopersici specifically attacks tomato plants and causes systemic disease of the root and stem.